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本文引用的文献

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Genetic analysis using random amplified polymorphic DNA markers.使用随机扩增多态性DNA标记进行遗传分析。
Methods Enzymol. 1993;218:704-40. doi: 10.1016/0076-6879(93)18053-f.
2
The emergence of Escherichia coli O157:H7 infection in the United States. The changing epidemiology of foodborne disease.美国大肠杆菌O157:H7感染的出现。食源性疾病不断变化的流行病学。
JAMA. 1993 May 5;269(17):2264-6.
3
RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains.随机扩增多态性DNA（任意引物）聚合酶链反应在区分相关细菌菌株方面比多位点酶电泳更为灵敏。
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4
Study of the influence of plasmids on the arbitrary primer polymerase chain reaction fingerprint of Escherichia coli strains.质粒对大肠杆菌菌株任意引物聚合酶链反应指纹图谱影响的研究
FEMS Microbiol Lett. 1994 Jan 15;115(2-3):335-9. doi: 10.1111/j.1574-6968.1994.tb06660.x.
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Magnetic separation techniques in diagnostic microbiology.诊断微生物学中的磁分离技术。
Clin Microbiol Rev. 1994 Jan;7(1):43-54. doi: 10.1128/CMR.7.1.43.
6
Immunomagnetic separation as a sensitive method for isolating Escherichia coli O157 from food samples.免疫磁珠分离法作为一种从食品样本中分离大肠杆菌O157的灵敏方法。
Epidemiol Infect. 1994 Aug;113(1):31-9. doi: 10.1017/s0950268800051438.
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DNA fingerprinting of medically important microorganisms by use of PCR.利用聚合酶链反应对具有医学重要性的微生物进行DNA指纹分析。
Clin Microbiol Rev. 1994 Apr;7(2):174-84. doi: 10.1128/CMR.7.2.174.
8
A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faeces.免疫磁珠分离法与直接培养法用于从牛粪中分离产志贺毒素大肠杆菌O157的比较。
J Med Microbiol. 1994 Jun;40(6):424-7. doi: 10.1099/00222615-40-6-424.
9
Molecular epidemiology of Escherichia coli O157:H7 strains by bacteriophage lambda restriction fragment length polymorphism analysis: application to a multistate foodborne outbreak and a day-care center cluster.通过噬菌体λ限制性片段长度多态性分析对大肠杆菌O157:H7菌株进行分子流行病学研究：应用于多州食源性疾病暴发和日托中心聚集性病例
J Clin Microbiol. 1993 Dec;31(12):3179-83. doi: 10.1128/jcm.31.12.3179-3183.1993.
10
Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing.通过脉冲场凝胶电泳和噬菌体分型对多州食源性大肠杆菌O157:H7疫情进行实验室调查。
J Clin Microbiol. 1994 Dec;32(12):3013-7. doi: 10.1128/jcm.32.12.3013-3017.1994.

使用煮沸培养物的模板对大肠杆菌O157:H7菌株进行任意引物PCR DNA指纹分析。

Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures.

作者信息

Madico G, Akopyants N S, Berg D E

机构信息

Departments of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA.

出版信息

J Clin Microbiol. 1995 Jun;33(6):1534-6. doi: 10.1128/jcm.33.6.1534-1536.1995.

DOI:10.1128/jcm.33.6.1534-1536.1995
PMID:7650181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228210/
Abstract

Arbitrarily primed PCR allows genetically different bacterial strains to be distinguished with great sensitivity and efficiency. We report that informative, reproducible arbitrarily primed PCR profiles can be obtained from Escherichia coli O157:H7 strains by using boiled stationary-phase cultures, without the need for time-consuming phenol extraction. This simple template preparation procedure should be especially useful in large epidemiologic studies when many strains must be typed.

摘要

任意引物PCR能够以高灵敏度和高效率区分基因不同的细菌菌株。我们报告称，通过使用煮沸的稳定期培养物，无需耗时的酚提取，即可从大肠杆菌O157:H7菌株中获得信息丰富、可重复的任意引物PCR图谱。在许多菌株必须进行分型的大型流行病学研究中，这种简单的模板制备方法应该特别有用。