使用煮沸培养物的模板对大肠杆菌O157:H7菌株进行任意引物PCR DNA指纹分析。
Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures.
作者信息
Madico G, Akopyants N S, Berg D E
机构信息
Departments of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110, USA.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1534-6. doi: 10.1128/jcm.33.6.1534-1536.1995.
Abstract
Arbitrarily primed PCR allows genetically different bacterial strains to be distinguished with great sensitivity and efficiency. We report that informative, reproducible arbitrarily primed PCR profiles can be obtained from Escherichia coli O157:H7 strains by using boiled stationary-phase cultures, without the need for time-consuming phenol extraction. This simple template preparation procedure should be especially useful in large epidemiologic studies when many strains must be typed.
摘要
任意引物PCR能够以高灵敏度和高效率区分基因不同的细菌菌株。我们报告称，通过使用煮沸的稳定期培养物，无需耗时的酚提取，即可从大肠杆菌O157:H7菌株中获得信息丰富、可重复的任意引物PCR图谱。在许多菌株必须进行分型的大型流行病学研究中，这种简单的模板制备方法应该特别有用。
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