Yamada M, Komatsu N, Okada K, Kato T, Miyazaki H, Miura Y
Department of Medicine, Jichi Medical School, Tochigi-ken, Japan.
Biochem Biophys Res Commun. 1995 Dec 5;217(1):230-7. doi: 10.1006/bbrc.1995.2768.
Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.
血小板生成素（TPO）是一种细胞因子，它能够支持巨核细胞祖细胞的增殖和分化以及巨核细胞的成熟。我们在此表明，丝裂原活化蛋白（MAP）激酶Erk1和Erk2参与了人TPO依赖的巨核细胞系UT-7/TPO中的TPO信号转导。TPO以剂量和时间依赖性方式诱导Erk1和Erk2蛋白的酪氨酸磷酸化。此外，MAP激酶的激活实际上是由TPO诱导的。这些结果表明，MAP激酶激活参与了TPO的信号通路，就像它参与其他细胞因子（如促红细胞生成素）的信号通路一样。
