Kwak J Y, Beaman K D, Gilman-Sachs A, Ruiz J E, Schewitz D, Beer A E
Department of Obstetrics and Gynecology, Finch University of Health Sciences/The Chicago Medical School, Illinois 60064, USA.
Am J Reprod Immunol. 1995 Aug;34(2):93-9. doi: 10.1111/j.1600-0897.1995.tb00924.x.
To analyze immunophenotypic profiles of peripheral blood and humoral autoimmune responses in women with a history of recurrent spontaneous abortions (RSA).
Peripheral blood lymphocyte subsets by flow cytometry and autoantibodies to phospholipids and nuclear components by ELISA were measured in nonpregnant and pregnant women with RSA of unknown etiology. Thirty-five pregnant and eighty-one nonpregnant women with RSA were studied. Seventeen nonpregnant and twenty-two pregnant normal controls were included.
Natural killer (NK) cells (CD56+) were significantly elevated in nonpregnant women with RSA as compared with nonpregnant controls. Pregnant women with RSA demonstrated significantly increased NK (CD56+, CD56+/CD16+) and B cells (CD19+) as compared with pregnant controls. Women who miscarried the index pregnancy demonstrated significantly lower CD3+ cells in comparison with normal controls. Women with RSA and antiphospholipid antibodies showed significantly elevated NK cells when compared with women without antiphospholipid antibodies. Women with autoantibodies to nuclear components demonstrated significantly elevated CD19+/CD5+ cells when compared to women without autoantibodies to nuclear components.
Women with RSA demonstrate an abnormal cellular immune response by increasing peripheral natural killer cells and B cells as compared with normal controls.
分析有复发性自然流产（RSA）病史女性的外周血免疫表型谱及体液自身免疫反应。
对病因不明的RSA非孕及孕龄女性，通过流式细胞术检测外周血淋巴细胞亚群，采用酶联免疫吸附测定（ELISA）检测抗磷脂及核成分自身抗体。研究了35例RSA孕龄女性和81例RSA非孕女性。纳入17例非孕及22例孕龄正常对照。
与非孕对照相比，RSA非孕女性的自然杀伤（NK）细胞（CD56+）显著升高。与孕龄对照相比，RSA孕龄女性的NK（CD56+、CD56+/CD16+）和B细胞（CD19+）显著增加。与正常对照相比，本次妊娠流产的女性CD3+细胞显著降低。与无抗磷脂抗体的女性相比，有RSA且有抗磷脂抗体的女性NK细胞显著升高。与无核成分自身抗体的女性相比，有核成分自身抗体的女性CD19+/CD5+细胞显著升高。
与正常对照相比，RSA女性通过增加外周自然杀伤细胞和B细胞表现出异常的细胞免疫反应。
