Suh S I, Baek W K, Park J W, Bae O S, Suh M H, Choe B K
Department of Microbiology, School of Medicine, Keimyung University, Taegu, Korea.
J Korean Med Sci. 1995 Jun;10(3):169-75. doi: 10.3346/jkms.1995.10.3.169.
CD44 is a glycoprotein expressed in a wide variety of cell types. Recently expression of some alternatively-spliced variants of CD44 transcripts (CD44v) has been suggested to play a potential role in tumor metastasis and the detection of CD44v containing exon 6 to 11 may be helpful for the diagnosis of cancers. Expressions of CD44v containing exon 6 to 11 were investigated in 20 human colorectal cancer samples, peripheral blood leukocytes isolated from colorectal cancer patients, and 4 colorectal cancer cell lines using reverse transcription-polymerase chain reaction and Southern blot analysis. The standard form of CD44 transcripts was expressed in all samples tested. CD44v containing exon 6 to 11 was expressed in 18 cases of colorectal cancers (sensitivity = 90%), 3 out of 4 cell lines, and one normal tissue (specificity = 95%). These results suggest that the expression of CD44v containing exon 6 to 11 can be regarded as tumor specific and that this marker may be helpful for the early diagnosis of colon cancers, if specimens from the early stage are available.
CD44是一种在多种细胞类型中表达的糖蛋白。最近，有研究表明，CD44转录本的一些可变剪接变体（CD44v）的表达可能在肿瘤转移中发挥潜在作用，检测包含外显子6至11的CD44v可能有助于癌症的诊断。使用逆转录-聚合酶链反应和Southern印迹分析，对20例人类结直肠癌样本、从结直肠癌患者分离的外周血白细胞以及4种结直肠癌细胞系中包含外显子6至11的CD44v的表达进行了研究。在所有测试样本中均表达了CD44转录本的标准形式。包含外显子6至11的CD44v在18例结直肠癌（敏感性=90%）、4种细胞系中的3种以及1个正常组织中表达（特异性=95%）。这些结果表明，包含外显子6至11的CD44v的表达可被视为肿瘤特异性的，并且如果有早期阶段的样本，该标志物可能有助于结肠癌的早期诊断。
