Kan M, Furukawa A, Aki M, Kanayama H, Kagawa S
Department of Urology, University of Tokushima, Japan.
Int J Urol. 1995 Nov;2(5):295-301.
Recently, it was noted that CD44 participates in a wide variety of cell-cell or cell-matrix interactions including tumor metastasis and the altered expression of CD44 splice variants is helpful in the diagnosis of colon and breast cancer. The purpose of the study was to investigate altered expression of CD44 in bladder cancer.
Thirty-three surgical specimens of 22 patients and seven bladder cancer cell-lines were analyzed by various methods including RT-PCR-Southern blotting. Northern blotting, and flow-cytometric analysis.
In RT-PCR analysis, the hematopoietic variant and numerous additional variants were detected in all cell-lines and almost all surgical specimens. In three out of seven cell-lines of bladder cancer (42.9%), especially numerous larger bands (ladder pattern) were detected using RT-PCR. These three cell-lines expressed a smaller quantity of CD44 mRNAs and CD44 molecules than the other four cell-lines. In eighteen out of 22 (81.8%) bladder cancer specimens, we detected larger variants that exceed 1500 base pairs, compared with the detection in four out of eleven (36.4%) visually normal mucosa specimens (P < 0.05). However, there was no correlation between the expression of the larger variants and clinicopathological features.
The quantitative and qualitative change of CD44 variants were demonstrated in bladder cancer.
最近，有研究指出CD44参与多种细胞间或细胞与基质间的相互作用，包括肿瘤转移，且CD44剪接变体的表达改变有助于结肠癌和乳腺癌的诊断。本研究旨在探讨膀胱癌中CD44的表达改变。
采用包括逆转录聚合酶链反应- Southern印迹法、Northern印迹法和流式细胞术分析等多种方法，对22例患者的33份手术标本和7种膀胱癌细胞系进行分析。
在逆转录聚合酶链反应分析中，所有细胞系和几乎所有手术标本中均检测到造血变体和许多其他变体。在7种膀胱癌细胞系中的3种（42.9%）中，尤其是使用逆转录聚合酶链反应检测到大量更大的条带（梯状模式）。这3种细胞系表达的CD44 mRNA和CD44分子数量少于其他4种细胞系。在22份膀胱癌标本中的18份（81.8%）中，我们检测到超过1500个碱基对的更大变体，而在11份肉眼正常黏膜标本中的4份（36.4%）中也检测到了这种变体（P < 0.05）。然而，更大变体的表达与临床病理特征之间没有相关性。
膀胱癌中CD44变体存在定量和定性变化。
