Temmerman M, Nagelkerke N, Bwayo J, Chomba E N, Ndinya-Achola J, Piot P
Department of Obstetrics and Gynaecology, University of Ghent, Belgium.
AIDS. 1995 Sep;9(9):1057-60.
To assess changes in the proportion of CD4 and CD8 T-lymphocyte profiles during pregnancy, at delivery and postpartum, and to determine whether HIV-1 infection affects the normal profile.
A total of 416 pregnant HIV-1-infected women and an age and parity-matched HIV-seronegative group of 407 pregnant women were enrolled into a prospective study on the impact of HIV-1 infection on pregnancy. Maternal blood was obtained for lymphocyte subset determination at enrollment, delivery and 6 weeks postpartum. Whole blood sample drawn in EDTA-containing tubes were used to determine T-helper/inducer (CD4) and T-suppressor/cytotoxic (CD8) cells by direct immunofluorescence using monoclonal antibodies.
No relationship was found between gestational age and any immunological variable. The CD4 percentage was lower postpartum than antenatally, in both HIV-1-seropositive and seronegative women, but this was not true for absolute CD4 counts. CD8 absolute counts and percentages were significantly higher postpartum than antenatally. The differences between HIV-1-seropositive and seronegative women in changes over pregnancy in CD4 and CD8 cells and their ratio, were not statistically significant.
Our findings do not support a short-term synergistic effect of HIV-1 and pregnancy on the immune function as determined by T-lymphocyte subsets.
评估孕期、分娩时及产后CD4和CD8 T淋巴细胞谱比例的变化，并确定HIV-1感染是否会影响正常谱。
共有416名感染HIV-1的孕妇和407名年龄及产次匹配的HIV血清阴性孕妇被纳入一项关于HIV-1感染对妊娠影响的前瞻性研究。在入组时、分娩时及产后6周采集孕妇血液用于淋巴细胞亚群测定。采集于含乙二胺四乙酸（EDTA）试管中的全血样本，使用单克隆抗体通过直接免疫荧光法测定辅助性/诱导性T细胞（CD4）和抑制性/细胞毒性T细胞（CD8）。
未发现胎龄与任何免疫变量之间存在关联。在HIV-1血清阳性和血清阴性的女性中，产后CD4百分比均低于产前，但CD4绝对计数并非如此。产后CD8绝对计数及百分比均显著高于产前。HIV-1血清阳性和血清阴性女性在孕期CD4和CD8细胞及其比例变化方面的差异无统计学意义。
我们的研究结果不支持HIV-1与妊娠对由T淋巴细胞亚群所确定的免疫功能存在短期协同效应。
