Arana-Chavez V E, Soares A M, Katchburian E
Department of Histology and Embriology, University of São Paulo, Brazil.
Arch Histol Cytol. 1995 Aug;58(3):285-92. doi: 10.1679/aohc.58.285.
Although intercellular junctions have been described between mature lamellar bone cells, little has been known about junctions between osteoblasts in early developing bone. We therefore conducted a freeze-fracture and ultrathin section study on developing calvaria of rat embryos aged 17-19 days to determine what types of intercellular junctions appear between osteoblasts in early osteogenesis. We observed that three main types of junctional structures, i.e., adherens of the macular type, gap, and focal tight junctions, coexist between osteoblasts in early developing bone. Their possible involvement in early morphogenetic events is discussed. Tight junctions are considered to be involved in compartmentalization of the early matrix and final polarization of osteoblasts.
尽管已对成熟板层骨细胞之间的细胞间连接进行了描述，但对于早期发育骨中破骨细胞之间的连接却知之甚少。因此，我们对17 - 19日龄大鼠胚胎发育中的颅骨进行了冷冻断裂和超薄切片研究，以确定在早期骨生成过程中破骨细胞之间出现何种类型的细胞间连接。我们观察到，在早期发育骨的破骨细胞之间共存着三种主要类型的连接结构，即斑状黏附连接、缝隙连接和局灶紧密连接。讨论了它们可能参与早期形态发生事件的情况。紧密连接被认为与早期基质的分隔和成骨细胞的最终极化有关。
