巯基嘌呤代谢物在按年龄分级的红细胞中的积累。
The accumulation of mercaptopurine metabolites in age fractionated red blood cells.
作者信息
Rostami-Hodjegan A, Lennard L, Lilleyman J S
机构信息
University of Sheffield Department of Medicine and Pharmacology, Royal Hallamshire Hospital, UK.
出版信息
Br J Clin Pharmacol. 1995 Sep;40(3):217-22. doi: 10.1111/j.1365-2125.1995.tb05776.x.
Abstract
- Red blood cells from four children with lymphoblastic leukemia were age fractionated on Percoll density gradients into 'young', 'middle-aged' and 'old' cells. 2. The rates of accumulation of the mercaptopurine (MP) metabolites thioguanine nucleotides (TGNs) and methylmercaptopurine nucleotides (MeMPs) were measured in the cell fractions from the start of MP continuing chemotherapy. 3. TGNs and MeMP metabolites were present in all the red cell fractions after 3 days oral MP. There was no significant difference between the metabolite concentrations measured in either young, middle-aged or old cells (Mann-Whitney P = 1.0 to 0.12). 4. These observations suggest that MP metabolites do not enter red cells at the stem cell level at the start of therapy. 5. With respect to the monitoring of therapy, these results suggest that the concentration of TGNs after 7 to 10 days MP could be used to predict eventual steady-state concentrations using a simple model.
摘要
- 从4名淋巴细胞白血病患儿的红细胞中，通过Percoll密度梯度法将其按年龄分为“年轻”、“中年”和“老年”细胞。2. 从巯嘌呤（MP）持续化疗开始，测定细胞组分中巯嘌呤（MP）代谢产物硫鸟嘌呤核苷酸（TGNs）和甲基巯嘌呤核苷酸（MeMPs）的积累速率。3. 口服MP 3天后，所有红细胞组分中均存在TGNs和MeMP代谢产物。在年轻、中年或老年细胞中测得的代谢产物浓度之间无显著差异（曼-惠特尼检验P = 1.0至0.12）。4. 这些观察结果表明，在治疗开始时，MP代谢产物不会在干细胞水平进入红细胞。5. 关于治疗监测，这些结果表明，使用简单模型，MP治疗7至10天后的TGNs浓度可用于预测最终稳态浓度。
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