Sharara A M, Higham M A, Spanevello A, Ind P W
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Br J Clin Pharmacol. 1995 Sep;40(3):283-5. doi: 10.1111/j.1365-2125.1995.tb05787.x.
Atrial natriuretic peptide (ANP) causes mast cell degranulation in rats in vivo and in vitro but is bronchodilator in humans. The aim of this study was to investigate the wheal and flare dose-response to intradermal injection of alpha-human ANP in normal humans. Eight normal subjects received five 30 microliters injections containing 1, 10, 39, 78, 117 pmol ANP and one each of normal saline, histamine 675 pmol and substance P 30 pmol. Maximum ANP flare response was greater but not significantly than that to saline at 1.55 +/- 0.6 (mean +/- s.e. mean) compared with 0.42 +/- 0.17 cm2, but much less than to histamine 9.86 +/- 0.97 or to substance P 12.5 +/- 1.2. Maximum ANP wheal response was significantly greater than that to saline at 0.38 +/- 0.08 compared with 0.18 +/- 0.05 cm2 (difference between means 0.20, 95% CI 0.05, 0.35), but much less than to histamine 0.75 +/- 0.06 or to substance P 1.05 +/- 0.08 cm2. No dose-response to ANP was demonstrated, though responses to the highest dose differed significantly from those to the lowest dose studied. We conclude that human cutaneous responses to ANP differ from those of animals and that the skin is less responsive than other tissues in humans.
心房利钠肽（ANP）在体内和体外均可引起大鼠肥大细胞脱颗粒，但对人类却是支气管扩张剂。本研究的目的是调查正常人体内皮内注射α-人ANP后的风团和潮红剂量反应。8名正常受试者接受了5次30微升的注射，其中分别含有1、10、39、78、117皮摩尔的ANP，以及一次生理盐水、675皮摩尔组胺和30皮摩尔P物质的注射。与生理盐水的0.42±0.17平方厘米相比，ANP的最大潮红反应更大，但无显著差异，为1.55±0.6（平均值±标准误平均值），但远小于组胺的9.86±0.97或P物质的12.5±1.2。ANP的最大风团反应显著大于生理盐水的反应，分别为0.38±0.08和0.18±0.05平方厘米（平均值之差为0.20，95%可信区间为0.05，0.35），但远小于组胺的0.75±0.06或P物质的1.05±0.08平方厘米。尽管对最高剂量的反应与所研究的最低剂量的反应有显著差异，但未显示出对ANP的剂量反应。我们得出结论，人类皮肤对ANP的反应与动物不同，并且在人类中皮肤的反应性低于其他组织。
