Lefebvre R, Chenoweth P J, Drost M, LeClear C T, MacCubbin M, Dutton J T, Suarez S S
Department of Anatomy, Cornell University, College of Veterinary Medicine, Ithaca, New York, USA.
Biol Reprod. 1995 Nov;53(5):1066-74. doi: 10.1095/biolreprod53.5.1066.
A reservoir for sperm has been found in the oviductal isthmus in several species. Sperm are apparently trapped in the reservoir by binding to the oviductal epithelium, although other factors may be involved. We hypothesized that binding sites for bovine sperm are limited to the isthmus and are regulated by the hormonal state of the cow. Ipsilateral oviducts were obtained from heifers that were preovulatory (in estrus), had ovulated recently (within 12 h), or were in diestrus (Day 10). The isthmic and the ampullar epithelium were milked out and incubated separately in serum-free (SFRE-199-2) medium, at 39 degrees C in 5% CO2. Frozen-thawed sperm from bulls were added to the epithelium and coincubated for 15 min. The number of spermatozoa that bound to explants was not affected by stage of cycle or by anatomic origin of the explants (p > 0.05). In an additional experiment, oviducts were infused with sperm in vivo and then prepared for scanning electron microscopy, which revealed that sperm were associated with ciliated epithelium in both the isthmus and ampulla. Thus, bovine sperm may form a reservoir in the isthmic end of the oviduct because it is the first oviductal region that they encounter.
在多个物种的输卵管峡部发现了精子储存库。精子显然是通过与输卵管上皮结合而被困在储存库中，不过可能还涉及其他因素。我们推测，牛精子的结合位点仅限于峡部，且受母牛激素状态的调节。从处于排卵前（发情期）、最近已排卵（12小时内）或处于发情间期（第10天）的小母牛身上获取同侧输卵管。将峡部和壶腹部上皮挤出，分别在无血清（SFRE - 199 - 2）培养基中于39℃、5%二氧化碳条件下孵育。将公牛的冻融精子加入上皮中，共同孵育15分钟。与外植体结合的精子数量不受周期阶段或外植体解剖来源的影响（p>0.05）。在另一项实验中，给输卵管进行体内精子灌注，然后制备用于扫描电子显微镜观察，结果显示精子与峡部和壶腹部的纤毛上皮均有关联。因此，牛精子可能在输卵管峡部末端形成储存库，因为这是它们遇到的第一个输卵管区域。
