Suarez S, Redfern K, Raynor P, Martin F, Phillips D M
Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Biol Reprod. 1991 Jun;44(6):998-1004. doi: 10.1095/biolreprod44.6.998.
Ejaculated boar sperm were incubated with explants of porcine oviductal mucosa that had been dissected from the isthmic and ampullar regions of gilts. Sperm bound within minutes to the epithelial surfaces of the explants. Binding was not affected by region (isthmus or ampulla) nor day of estrous cycle (Day 0 or Day 10), but was increased by addition of 70 pg/ml 17 beta-estradiol to the medium. Scanning electron micrographs indicated that sperm bound, via the acrosomal region, to ciliated cells. After 24 h, the numbers of bound sperm dropped significantly, but the motility of the bound sperm did not. A mucous material that entrapped sperm was observed on the epithelial surfaces of 23/32 isthmic and only 4/32 ampullar explants. These results indicate that sperm sticking to ciliated cells and mucus can create a sperm reservoir in the isthmus, but the means by which sperm are released remain unknown.
将射出的公猪精子与从后备母猪的峡部和壶腹部区域解剖得到的猪输卵管黏膜外植体一起孵育。精子在几分钟内就与外植体的上皮表面结合。结合不受区域（峡部或壶腹部）或发情周期天数（第0天或第10天）的影响，但向培养基中添加70 pg/ml的17β-雌二醇会增加结合。扫描电子显微镜照片显示，精子通过顶体区域与纤毛细胞结合。24小时后，结合的精子数量显著下降，但结合精子的活力没有下降。在32个峡部外植体中的23个和仅32个壶腹部外植体中的4个的上皮表面观察到截留精子的黏液物质。这些结果表明，黏附在纤毛细胞和黏液上的精子可以在峡部形成一个精子库，但精子释放的方式仍然未知。
