Parveen S, Morshed S A, Nishioka M
Third Department of Internal Medicine, Kagawa Medical School, Japan.
J Gastroenterol Hepatol. 1995 Jul-Aug;10(4):438-45. doi: 10.1111/j.1440-1746.1995.tb01597.x.
The purpose of the present study is to evaluate the centromeric pattern on human laryngeal tumour (HEp-2) cells by indirect immunofluorescent (IIF) test and to compare their reactivities with a newly developed recombinant centromere protein B enzyme linked immunosorbent assay (CENP-B ELISA) test using sera of antinuclear antibody (ANA)-reactive primary biliary cirrhosis (PBC) patients. Antimitochondrial antibody (AMA) subtypes (PDC-E2, BCOADC-E2, OGDC, protein X, and PDC-E1 alpha) by Western blot were also investigated to see whether they have any effect on the expression of CENP-B reactivities. A centromeric pattern (anticentromere antibody [ACA]) was detected in 11 of 25 (44%) PBC patients whereas CENP-B reactivity was found in 15 (60%) of them. There were some differences in IIF patterns and CENP-B reactivities. One PBC serum with indistinguishable ANA pattern reacted with CENP-B. Eight of 15 (53%) CENP-B reactive patients had other autoimmune-like disorders. Of 181 healthy sera, none was reactive for ACA either by IIF or by ELISA test. There was a correlation between ACA IIF and CENP-B ELISA titres (r = 0.824, P < 0.001). However, no correlation was observed between either CENP-B or AMA reactivities and/or between either autoantibodies or laboratory and histologic indices of PBC. These findings suggest that recombinant CENP-B ELISA appears to be more sensitive in identifying ACA than IIF, underlying its potential value as a screening test for the diagnosis of PBC complicated with other autoimmune-like disorders. The presence of multiple autoantibodies in PBC sera may reflect heterogeneous antigens recognition, and requires further study to identify target antigens at cellular and molecular levels.
本研究的目的是通过间接免疫荧光（IIF）试验评估人喉肿瘤（HEp-2）细胞上的着丝粒模式，并使用抗核抗体（ANA）反应性原发性胆汁性肝硬化（PBC）患者的血清，将其反应性与新开发的重组着丝粒蛋白B酶联免疫吸附测定（CENP-B ELISA）试验进行比较。还通过蛋白质印迹法研究了抗线粒体抗体（AMA）亚型（丙酮酸脱氢酶复合物E2、二氢硫辛酸转琥珀酰酶E2、2-氧代戊二酸脱氢酶、蛋白X和丙酮酸脱氢酶E1α），以观察它们是否对着丝粒蛋白B反应性的表达有任何影响。在25例PBC患者中的11例（44%）检测到着丝粒模式（抗着丝粒抗体[ACA]），而在其中15例（60%）中发现了CENP-B反应性。IIF模式和CENP-B反应性存在一些差异。一份ANA模式无法区分的PBC血清与CENP-B发生反应。15例CENP-B反应性患者中有8例（53%）患有其他自身免疫样疾病。在181份健康血清中，通过IIF或ELISA试验均无ACA反应性。ACA IIF和CENP-B ELISA滴度之间存在相关性（r = 0.824，P < 0.001）。然而，未观察到CENP-B或AMA反应性之间以及自身抗体或PBC的实验室和组织学指标之间的相关性。这些发现表明，重组CENP-B ELISA在识别ACA方面似乎比IIF更敏感，这突出了其作为诊断合并其他自身免疫样疾病的PBC的筛查试验的潜在价值。PBC血清中多种自身抗体的存在可能反映了异质抗原识别，需要进一步研究以在细胞和分子水平上鉴定靶抗原。
