Malette B, Filion B, St-Jacques S, Kan F W, Bleau G
Department of Biochemistry, Université de Montréal, Quebec, Canada.
Microsc Res Tech. 1995 Aug 15;31(6):470-7. doi: 10.1002/jemt.1070310603.
In several mammalian species, the epithelial secretory cells of the oviduct synthesize and secrete specific glycoproteins that become associated with the zona pellucida of the ovulated oocyte. These glycoproteins are collectively designated as oviductins. A monoclonal antibody directed against hamster oviductin was used to study the ontogeny of this glycoprotein. Indirect immunofluorescence experiments performed on sections of hamster oviduct revealed that the glycoprotein begins to be secreted in 10-day-old females and that all of the oviductal secretory cells showed fluorescent staining by day 14. The intensity of the immunofluorescence reaction reached a maximum in the 28-day-old females. The oviducts of the 7-day-old hamster incorporated [35S]methionine in vitro into several proteins; however, the production and secretion of detectable amounts of radiolabeled oviductin only began at 14 days of age and reached a maximum at day 28 of age. It appears that the ontogeny of oviductin parallels the hormone dependent changes leading to sexual maturation and that its maximum secretion is already established at the time of the first ovulatory cycle. These results are substantiated by the fact that the production of oviductin is induced in estradiol-treated, but not progesterone or non-treated prepubertal animals, as determined by indirect immunofluorescence experiments.
在几种哺乳动物中，输卵管的上皮分泌细胞合成并分泌特定的糖蛋白，这些糖蛋白会与排卵的卵母细胞的透明带结合。这些糖蛋白统称为输卵管蛋白。一种针对仓鼠输卵管蛋白的单克隆抗体被用于研究这种糖蛋白的个体发生。对仓鼠输卵管切片进行的间接免疫荧光实验表明，这种糖蛋白在10日龄的雌性仓鼠中开始分泌，到14日龄时，所有输卵管分泌细胞都显示出荧光染色。免疫荧光反应的强度在28日龄的雌性仓鼠中达到最大值。7日龄仓鼠的输卵管在体外将[35S]甲硫氨酸掺入几种蛋白质中；然而，可检测量的放射性标记输卵管蛋白的产生和分泌仅在14日龄时开始，并在28日龄时达到最大值。输卵管蛋白的个体发生似乎与导致性成熟的激素依赖性变化平行，并且其最大分泌量在第一个排卵周期时就已确定。间接免疫荧光实验表明，雌激素处理的青春期前动物可诱导输卵管蛋白的产生，而孕酮处理或未处理的青春期前动物则不能，这一事实证实了这些结果。
