Wei Y, Quertermous T, Wagner T E
Molecular and Cellular Biology Program, Ohio University, Athens 45701, USA.
Stem Cells. 1995 Sep;13(5):541-7. doi: 10.1002/stem.5530130512.
Cultured murine yolk sac cells transfected with the cytomegalovirus immediate early promoter/human growth hormone (CMVIE-hGH) fusion gene, expressing high levels of hGH in culture, and suspended in Matrigel were subcutaneously (s.c.) injected into experimental mice. The injected cells were shown to form discrete vesicular structures within the Matrigel implant, suggesting directed differentiation of the embryonic yolk sac cells into endothelial tissue. Human growth hormone radioimmune assay of these mice showed sustained physiologically significant levels of hGH in their serum for beyond four months. These results confirmed that long-term cultured murine embryonic yolk sac cells can be induced to differentiate into endothelial cells both in vivo and in vitro and suggested a novel approach to the delivery to the circulation of therapeutic proteins for the treatment of inherited and acquired diseases.
用巨细胞病毒立即早期启动子/人生长激素（CMVIE-hGH）融合基因转染的培养鼠卵黄囊细胞，在培养中表达高水平的hGH，并悬浮于基质胶中，然后皮下（s.c.）注射到实验小鼠体内。注射的细胞在基质胶植入物内形成离散的囊泡结构，提示胚胎卵黄囊细胞定向分化为内皮组织。对这些小鼠进行的人生长激素放射免疫测定显示，其血清中hGH水平在四个多月的时间里持续保持在生理显著水平。这些结果证实，长期培养的鼠胚胎卵黄囊细胞在体内和体外均可被诱导分化为内皮细胞，并提示了一种向循环系统递送治疗性蛋白质以治疗遗传性和获得性疾病的新方法。
