Wei Y, Li J, Chen W Y, Wagner T E
Edison Biotechnology Institute, Molecular and Cellular Biology Program, Ohio University, Athens 45701, USA.
Stem Cells. 1997;15(5):364-7. doi: 10.1002/stem.150364.
Long-term cultured murine embryonic yolk sac cells that are capable of forming capillary structures when cultured on base membrane proteins (Matrigel) were successfully transfected with a human growth hormone antagonist (G120R) gene. Cells that stably express relatively high levels of G120R were co-implanted s.c. with Matrigel into BALB/c mice. G120R can be detected in the sera of those implanted mice for more than 14 days at levels from 4 ng/ml to 28 ng/ml. The insulin-like growth factor-1 levels in the sera of those implanted mice were significantly affected by the delivered G120R. One of the physiological effects of G120R delivered by this murine embryonic yolk sac cell-derived mini-organ system is to decrease the growth rate of the implanted mice. This gene delivery system can also be used as an alternative to transgenic animals to study protein function in vivo.
长期培养的小鼠胚胎卵黄囊细胞，当在基底膜蛋白（基质胶）上培养时能够形成毛细血管结构，已成功转染人生长激素拮抗剂（G120R）基因。稳定表达相对高水平G120R的细胞与基质胶一起皮下共植入BALB/c小鼠体内。在那些植入小鼠的血清中可检测到G120R超过14天，水平为4 ng/ml至28 ng/ml。所递送的G120R显著影响那些植入小鼠血清中的胰岛素样生长因子-1水平。由这种小鼠胚胎卵黄囊细胞衍生的微型器官系统递送的G120R的生理作用之一是降低植入小鼠的生长速率。这种基因递送系统也可作为转基因动物的替代物用于体内研究蛋白质功能。
