Patel B, Khaliq A, Jarvis-Evans J, Boulton M, Arrol S, Mackness M, McLeod D
Department of Ophthalmology, University of Manchester, UK.
Biochem Mol Biol Int. 1995 Jul;36(4):907-12.
Populations of cells within solid tumours are exposed to low oxygen concentrations. The mechanism by which tumour cells tolerate such hypoxia is unknown but it may parallel responses to other types of cellular stress. We investigated the effect of oxygen on steady state levels of inducible heat shock protein 70 mRNA in cultured human hepatoma cells. Northern blot analysis demonstrated that hypoxia increased HSP 70 mRNA levels within 3 hours, with a transient 12-fold increase at 6 hours compared with normoxia. We also showed that heat shock induced a 20-fold increase in HSP 70 mRNA. This data suggests that HSPs may be important in tumour progression by protecting cells from hypoxic stress.
实体肿瘤中的细胞群体暴露于低氧浓度环境。肿瘤细胞耐受这种低氧的机制尚不清楚，但可能与对其他类型细胞应激的反应相似。我们研究了氧对培养的人肝癌细胞中诱导型热休克蛋白70 mRNA稳态水平的影响。Northern印迹分析表明，低氧在3小时内增加了HSP 70 mRNA水平，与常氧相比，6小时时短暂增加了12倍。我们还表明，热休克诱导HSP 70 mRNA增加了20倍。这些数据表明，热休克蛋白可能通过保护细胞免受低氧应激在肿瘤进展中起重要作用。
