Thomas C M, Vos P, Zabeau M, Jones D A, Norcott K A, Chadwick B P, Jones J D
Sainsbury Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
Plant J. 1995 Nov;8(5):785-94. doi: 10.1046/j.1365-313x.1995.08050785.x.
Using the technique of amplified restriction fragment polymorphism (AFLP) analysis, and bulked segregant pools from F2 progeny of the cross Lycopersicon esculentum (Cf9) x L. pennellii, approximately 42,000 AFLP loci for tight linkage to the tomato Cf-9 gene for resistance to Cladosporium fulvum have been screened. Analysis of F2 recombinants identified three markers which co-segregated with Cf-9. The Cf-9 gene has recently been isolated by transposon tagging using the maize transposon Dissociation (Ds). Analysis of plasmid clones containing Cf-9 shows that two of these markers are located on opposite sides of the gene separated by 15.5 kbp of intervening DNA. AFLP analysis provides a rapid and efficient technique for detecting large numbers of DNA markers and should expedite plant gene isolation by positional cloning and the construction of high-density molecular linkage maps of plant genomes.
利用扩增限制性片段多态性（AFLP）分析技术，以及番茄（Cf9）与潘那利番茄杂交F2代子代的混合分离群体，已筛选出约42,000个AFLP位点，用于与番茄Cf-9基因紧密连锁，该基因赋予对番茄叶霉病的抗性。对F2重组体的分析鉴定出三个与Cf-9共分离的标记。最近通过使用玉米转座子解离（Ds）进行转座子标签法分离出了Cf-9基因。对含有Cf-9的质粒克隆的分析表明，其中两个标记位于该基因的两侧，中间间隔15.5千碱基对的间隔DNA。AFLP分析为检测大量DNA标记提供了一种快速有效的技术，应能通过定位克隆加速植物基因分离，并构建植物基因组的高密度分子连锁图谱。
