Dickinson M J, Jones D A, Jones J D
Sainsbury Laboratory, John Innes Centre for Plant Science Research, Norwich, England.
Mol Plant Microbe Interact. 1993 May-Jun;6(3):341-7. doi: 10.1094/mpmi-6-341.
Analysis of restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers in tomato plants segregating for resistance to the fungus Cladosporium fulvum was used to localize the resistance genes Cf-2 and Cf-5 to the same region of chromosome 6. This region, between GP79 and Aps-1, is the same as that reported for the Mi gene, which confers resistance to root-knot nematodes (meloidogyne spp.). Recombination values based on F2 populations from crosses between near-isogenic lines of L. esculentum 'Moneymaker' carrying Cf-2 or Cf-5 and Lycopersicon pennellii, indicate that this region occupies 4-5 centiMorgans (cM). However, in F2 populations from crosses between the L. esculentum stock LA1190 carrying yv and these lines, this value is 1-2 cM. The Cf-2 gene, introduced into L. esculentum from L. pimpinellifolium, is on an introgressed segment that extends from a point distal to GP79 to a point between TG232 and H2D1. The origin of Cf-5 was found to be L. esculentum var. cerasiforme rather than L. pimpinellifolium as previously reported. No RFLP markers and only one RAPD marker showed a polymorphism between Moneymaker and the near-isogenic line carrying Cf-5.
对番茄植株中与抗番茄叶霉病菌（Cladosporium fulvum）相关的限制性片段长度多态性（RFLP）和随机扩增多态性DNA（RAPD）标记进行分析，以将抗性基因Cf - 2和Cf - 5定位到第6号染色体的同一区域。该区域位于GP79和Aps - 1之间，与报道的赋予抗根结线虫（Meloidogyne spp.）能力的Mi基因所在区域相同。基于携带Cf - 2或Cf - 5的番茄‘Moneymaker’近等基因系与潘那利番茄（Lycopersicon pennellii）杂交产生的F2群体的重组值表明，该区域占4 - 5厘摩（cM）。然而，在携带yv的番茄品系LA1190与这些品系杂交产生的F2群体中，该值为1 - 2 cM。从醋栗番茄（L. pimpinellifolium）导入番茄的Cf - 2基因位于一个渐渗片段上，该片段从GP79远端的一点延伸到TG232和H2D1之间的一点。发现Cf - 5的起源是樱桃番茄（L. esculentum var. cerasiforme），而非先前报道的醋栗番茄。在‘Moneymaker’和携带Cf - 5的近等基因系之间，没有RFLP标记显示多态性，只有一个RAPD标记显示多态性。
