加倍单倍体水稻群体中AFLP标记的多态性、分布及分离情况
Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population.
作者信息
Maheswaran M, Subudhi P K, Nandi S, Xu J C, Parco A, Yang D C, Huang N
机构信息
International Rice Research Institute, P.O. Box 933, Manila, Philippines.
出版信息
Theor Appl Genet. 1997 Jan;94(1):39-45. doi: 10.1007/s001220050379.
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution and inheritance of AFLP markers with a doubled haploid rice population derived from 'IR64'/'Azucena'. Using only 20 pairs of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with AFLP markers they will be very useful for marker-assisted backcrossing.
我们利用新开发的扩增片段长度多态性（AFLP）技术，以来源于“IR64”/“Azucena”的加倍单倍体水稻群体为材料，研究AFLP标记的多态性、分布及遗传情况。仅使用20对引物组合，我们检测到945条AFLP条带，其中208条具有多态性。所有208个AFLP标记均被定位并分布于所有12条染色体上。将这些标记与该群体中已定位的RFLP标记进行比较时，我们发现AFLP标记在水稻中具有高度多态性，并遵循孟德尔分离规律。由于利用AFLP标记能够快速构建水稻连锁图谱，它们将在标记辅助回交中非常有用。
Zotero 插件