噬菌体展示：通过定向进化进行蛋白质工程。

Phage display: protein engineering by directed evolution.

作者信息

O'Neil K T, Hoess R H

机构信息

DuPont Merck Pharmaceutical Company, Chemical and Physical Sciences Department, Wilmington, DE 19880-0328, USA.

出版信息

Curr Opin Struct Biol. 1995 Aug;5(4):443-9. doi: 10.1016/0959-440x(95)80027-1.

DOI:10.1016/0959-440x(95)80027-1

PMID:8528759

Abstract

Phage display of proteins has become an important tool for protein engineering. Over the past year, the versatility of the technology has expanded to include the development of DNA-binding proteins with novel specificities, energetics of protein folding and directed evolution of antibodies. In addition, display of expressed cDNA libraries opens an exciting opportunity for studying protein-protein interactions.

摘要

蛋白质的噬菌体展示已成为蛋白质工程的一项重要工具。在过去的一年里，该技术的用途得到了扩展，包括开发具有新特异性的DNA结合蛋白、蛋白质折叠的能量学以及抗体的定向进化。此外，表达的cDNA文库的展示为研究蛋白质-蛋白质相互作用提供了一个令人兴奋的机会。