Reinhold D, Bank U, Bühling F, Lendeckel U, Ansorge S
Department of Internal Medicine, Otto von Guericke University Magdeburg, Germany.
J Interferon Cytokine Res. 1995 Aug;15(8):685-90. doi: 10.1089/jir.1995.15.685.
The multifunctional cytokine transforming growth factor-beta 1 (TGF-beta 1) is known to inhibit the proliferation of lymphocytes. Whether this effect is a result of a direct action of TGF-beta 1 or an involvement of other "immunoinhibitory" cytokines is not yet clear. Here we have analyzed the effects of TGF-beta 1 on IL-10 and IL-1RA production in pokeweed mitogen-stimulated peripheral blood mononuclear cells (PBMC) and purified T lymphocytes. We show in these systems that TGF-beta 1 at a concentration of 10 ng/ml significantly suppresses both IL-10 mRNA expression and IL-10 production. IL-2 and IL-6 were capable of abolishing the effect of TGF-beta 1 on DNA synthesis and production of IL-10 by T lymphocytes in an additive manner. However, TGF-beta 1 did not influence IL-1RA production in PWM-stimulated PBMC. The present data show that the inhibitory effect of TGF-beta 1 on mitogen-activated immune cells is not the consequence of induction of the inhibitory cytokines IL-10 or IL-1RA but rather an inhibitory action on the production of IL-2 and/or IL-6.
多功能细胞因子转化生长因子-β1（TGF-β1）已知可抑制淋巴细胞增殖。TGF-β1的这种作用是其直接作用的结果还是其他“免疫抑制”细胞因子参与其中尚不清楚。在此，我们分析了TGF-β1对商陆丝裂原刺激的外周血单个核细胞（PBMC）和纯化的T淋巴细胞中白细胞介素-10（IL-10）和白细胞介素-1受体拮抗剂（IL-1RA）产生的影响。我们在这些系统中表明，浓度为10 ng/ml的TGF-β1可显著抑制IL-10 mRNA表达和IL-10产生。白细胞介素-2（IL-2）和白细胞介素-6（IL-6）能够以累加方式消除TGF-β1对T淋巴细胞DNA合成和IL-10产生的影响。然而，TGF-β1并不影响PWM刺激的PBMC中IL-1RA的产生。目前的数据表明，TGF-β1对有丝分裂原激活的免疫细胞的抑制作用不是诱导抑制性细胞因子IL-10或IL-1RA的结果，而是对IL-2和/或IL-6产生的抑制作用。
