Reinhold D, Bank U, Bühling F, Lendeckel U, Ulmer A J, Flad H D, Ansorge S
Department of Internal Medicine, Medical Academy Magdeburg, Germany.
Cytokine. 1994 Jul;6(4):382-8. doi: 10.1016/1043-4666(94)90062-0.
The multifunctional cytokine transforming growth factor-beta 1 (TGF-beta 1) is known to inhibit the proliferation of lymphocytes. However, the role of TGF-beta 1 in the production and secretion of various interleukins is not yet clear. In this study we have analysed in parallel the effects of TGF-beta 1 on both DNA synthesis and production of the cytokines IL-1, IL-2, IL-6, and TNF-alpha by pokeweed mitogen-stimulated peripheral blood mononuclear cells. With this stimulation system we show that TGF-beta 1 at a concentration of 15 ng/ml significantly suppresses IL-2 and IL-6 production. The release of IL-1 and TNF-alpha, however, was not influenced under these conditions. Under similar conditions DNA synthesis of PWM-stimulated PBMC was found to be inhibited by 50 +/- 10%. Using flow cytometric methods we could demonstrate that TGF-beta 1 arrested the cells in the G0/G1 phase of the cell cycle. Taken together, these results suggest that TGF-beta 1 may suppress immune responses by inhibiting the endogenous production of IL-2 and IL-6.
多功能细胞因子转化生长因子β1（TGF-β1）已知可抑制淋巴细胞增殖。然而，TGF-β1在各种白细胞介素产生和分泌中的作用尚不清楚。在本研究中，我们同时分析了TGF-β1对商陆有丝分裂原刺激的外周血单个核细胞DNA合成以及细胞因子IL-1、IL-2、IL-6和TNF-α产生的影响。通过该刺激系统，我们发现浓度为15 ng/ml的TGF-β1可显著抑制IL-2和IL-6的产生。然而，在这些条件下，IL-1和TNF-α的释放未受影响。在类似条件下，发现商陆有丝分裂原刺激的外周血单个核细胞的DNA合成被抑制50±10%。使用流式细胞术方法，我们可以证明TGF-β1使细胞停滞在细胞周期的G0/G1期。综上所述，这些结果表明TGF-β1可能通过抑制IL-2和IL-6的内源性产生来抑制免疫反应。
