Gacko M, Głowiński S, Worowska A, Głowiński J, Zakrzewski M, Worowski K
Department of Vascular Surgery and Transplantology, Medical Academy of Bialystok.
Rocz Akad Med Bialymst. 1995;40(1):172-9.
The aim of the study was to evaluate the enzyme activity of cellular membranes (GGT), cytosol (ALT, AST) and lysosome (AP, AcP) in the cytosol, whole homogenate and blood serum during declamping shock, following release of abdominal aorta cross-clamping. The aorta was clamped for 60 minutes. An increase in GGT, AP and AcP activities in the cytosol and whole homogenate of the renal cortex, renal medulla, liver, lung, heart and the skeletal muscle occurs after declamping. Rise in the enzymatic activity, especially of acid phosphatase is higher when the aorta above renal arteries was clamped. However, its activity in the blood serum remains unchanged. Alterations in the distribution and the activity of the studied enzymes may indicate that aortic clamping damages the endoplasmic reticulum and lysosomal membranes. Yet, cellular membranes preserve their structural and functional integrity.
本研究的目的是评估在腹主动脉交叉夹闭解除后发生的夹闭性休克期间，细胞溶质、全匀浆和血清中细胞膜（γ-谷氨酰转移酶）、细胞溶质（谷丙转氨酶、谷草转氨酶）和溶酶体（碱性磷酸酶、酸性磷酸酶）的酶活性。主动脉夹闭60分钟。夹闭解除后，肾皮质、肾髓质、肝脏、肺、心脏和骨骼肌的细胞溶质和全匀浆中的γ-谷氨酰转移酶、碱性磷酸酶和酸性磷酸酶活性增加。当肾动脉上方的主动脉被夹闭时，酶活性的升高，尤其是酸性磷酸酶的升高更为明显。然而，其在血清中的活性保持不变。所研究酶的分布和活性变化可能表明主动脉夹闭会损伤内质网和溶酶体膜。然而，细胞膜保持其结构和功能完整性。
