Gacko M, Głowiński J, Skrzydlewska E, Worowska A, Głowiński S
Department of Vascular Surgery and Transplantology, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1996;41(2):341-6.
Acidification to pH 5.0 of various organ homogenates prepared in 0.25 M sucrose causes aggregation of cell organelles. Aggregated organelles are removed through standard centrifugation. Cytosol obtained reveals only slight activity of membrane and lysosomal enzymes. The cytosol is useful for evaluation of changes in enzyme distribution in cell.
将在0.25M蔗糖中制备的各种器官匀浆酸化至pH 5.0会导致细胞器聚集。聚集的细胞器通过标准离心去除。得到的胞质溶胶仅显示出轻微的膜和溶酶体酶活性。该胞质溶胶可用于评估细胞中酶分布的变化。
