Kim H C, Lee Y S, Furukawa F, Nishikawa A, Takahashi M
Toxicology Research Center, Korea Research Institute of Chemical Technology, Yusongku, Daejon, South Korea.
Cancer Lett. 1995 Nov 27;98(1):71-6.
This study was performed for developing a new medium-term carcinogenicity bioassay treated with D-galactosamine (DGA) as a non-surgical method without partial hepatectomy (PH). In male F344 rats initiated with diethylnitrosamine (DEN, 200 mg/kg i.p.), enhancing effects of DGA (300 mg/kg i.p.) given twice 3 weeks apart during the promotion procedure with 2-acetylaminofluorene (2-AAF, 0.01% in diet) were compared along with those of PH by analyzing preneoplastic glutathione S-transferase placental form positive (GST-P+) hepatocyte foci as endpoint marker lesions. The DGA treatment did not affect the body weight gain whereas the PH treatment caused a transient body weight loss. Although both bioassay protocols were effective to detect the potential hepatocarcinogenicity of 2-AAF, the number and area of GST-P positive foci per cm2 were larger in the bioassay using DGA than in that using PH, the number being statistically significant (P < 0.05). Our results thus suggest that the present bioassay protocol with repeated administration of DGA instead of PH may offer a new and sensitive method to screen large numbers of environmental carcinogens.
本研究旨在开发一种新的中期致癌性生物测定方法，该方法采用D - 半乳糖胺（DGA）处理，作为一种无需部分肝切除术（PH）的非手术方法。在经二乙基亚硝胺（DEN，200mg/kg腹腔注射）启动的雄性F344大鼠中，以2 - 乙酰氨基芴（2 - AAF，饲料中含0.01%）进行促癌过程中，比较间隔3周两次腹腔注射DGA（300mg/kg）的增强作用与PH的增强作用，通过分析癌前谷胱甘肽S - 转移酶胎盘型阳性（GST - P +）肝细胞灶作为终点标记病变。DGA处理不影响体重增加，而PH处理导致体重短暂下降。虽然两种生物测定方案都能有效检测2 - AAF的潜在肝致癌性，但使用DGA的生物测定中每平方厘米GST - P阳性灶的数量和面积大于使用PH的生物测定，数量具有统计学显著性（P < 0.05）。因此，我们的结果表明，本研究中采用重复给予DGA替代PH的生物测定方案可能为筛选大量环境致癌物提供一种新的、灵敏的方法。
