Tsuda H, Masui T, Ikawa E, Imaida K, Ito N
First Department of Pathology, Nagoya City University Medical School, Japan.
Cancer Lett. 1987 Oct 30;37(2):163-71. doi: 10.1016/0304-3835(87)90159-5.
Effectiveness of two different chemically induced stimuli for hepatocellular proliferation was compared with regard to that of commonly used partial hepatectomy (PH), for the purpose of developing short-term protocol for the assay of promoting agents of hepatocarcinogenesis. Enhancing effect of D-galactosamine (DGA) and carbon tetrachloride (CCl4) given during the promotion procedure by 2-acetylaminofluorene (2-AAF) was compared along with PH in rats initiated by diethylnitrosamine (DEN), using preneoplastic glutathione S-transferase positive (GST-P+) hepatocyte foci as an end-point marker lesion. The number of GST-P+ foci per cm2 was largest in the group given CCl4 followed by DGA, no treatment (2-AAF alone) and PH. In contrast, the area (mm2) per cm2 and mean diameter of the focus were largest in the PH group then DGA followed by CCl4 and no treatment. The results indicate that the number of GST-P+ foci were not clearly affected by 3 different treatments whereas area and size of foci which represented the result of promoting effect were clearly influenced by those treatments, indicating they caused differential proliferation of initiated cells. In this respect, even though PH is the most potent procedure, DGA is also efficient and preferred to CCl4 for the non-surgical enhancing method.
为了开发一种用于检测肝癌发生促进剂的短期实验方案，比较了两种不同化学诱导刺激对肝细胞增殖的有效性与常用的部分肝切除术（PH）的有效性。在由二乙基亚硝胺（DEN）引发的大鼠中，以癌前谷胱甘肽S-转移酶阳性（GST-P+）肝细胞灶作为终点标记病变，比较了在由2-乙酰氨基芴（2-AAF）进行的促进过程中给予D-半乳糖胺（DGA）和四氯化碳（CCl4）的增强效果以及PH的增强效果。每平方厘米GST-P+灶的数量在给予CCl4的组中最多，其次是DGA、未处理组（仅2-AAF）和PH组。相反，每平方厘米的面积（平方毫米）和灶的平均直径在PH组中最大，其次是DGA，然后是CCl4和未处理组。结果表明，3种不同处理对GST-P+灶的数量没有明显影响，而代表促进效果的灶的面积和大小则明显受到这些处理的影响，表明它们导致起始细胞的增殖存在差异。在这方面，尽管PH是最有效的方法，但对于非手术增强方法，DGA也是有效的，并且比CCl4更可取。
