Lin K H, Lin Y W, Lee H F, Liu W L, Chen S T, Chang K S, Cheng S Y
Graduate Institute of Clinical Medicine, Chang Gung College of Medicine and Technology, Kwei-san, Taoyuan-Hsien, Taiwan, Republic of China.
Cancer Lett. 1995 Nov 27;98(1):89-95.
To understand the role of thyroid hormone in metastasis, we studied the expression of the anti-metastatic nm23 gene in eight human hepatocellular carcinoma (HCC) cell lines. These cells differentially expressed the anti-metastatic nm23 gene. A low level of nm23 proteins was found to have a high in vitro invasive activity which correlated closely with an overexpression of the thyroid hormone beta 1 nuclear receptor (h-TR beta 1). Concurrent with the down-regulation of h-TR beta 1, the invasive activity of HCC cells was suppressed by the thyroid hormone, 3,3',5-triiodo-L-thyronine (T3). These results indicate that the invasive activity of HCC cells was regulated by T3, suggesting that T3 could be involved in modulating the functions of nm23.
为了解甲状腺激素在转移中的作用，我们研究了抗转移nm23基因在8种人肝癌（HCC）细胞系中的表达。这些细胞差异表达抗转移nm23基因。发现低水平的nm23蛋白具有高体外侵袭活性，这与甲状腺激素β1核受体（h-TRβ1）的过表达密切相关。随着h-TRβ1的下调，甲状腺激素3,3',5-三碘-L-甲状腺原氨酸（T3）抑制了肝癌细胞的侵袭活性。这些结果表明，肝癌细胞的侵袭活性受T3调节，提示T3可能参与调节nm23的功能。
