窝仔数对新生大鼠正常视网膜血管发育的影响。

The effect of litter size on normal retinal vascular development in the neonatal rat.

作者信息

Holmes J M, Duffner L A

机构信息

Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.

出版信息

Curr Eye Res. 1995 Aug;14(8):737-40. doi: 10.3109/02713689508998503.

DOI:10.3109/02713689508998503

PMID:8529411

Abstract

Many animal models of retinal disease use the neonatal rat. Raising rat pups in large litters has been shown to result in postnatal growth retardation. We investigated the effect of litter size on the normal postnatal vascularization of the neonatal rat retina. Sixty-six newborn rat pups were divided among 5 nursing mothers into 3 small litters (n = 10) and 2 large litters (n = 18). On day 6 of life the rats were sacrificed and total retinal and vascularized retinal areas analyzed. The total retinal area was reduced in the rats raised in larger litters (28.6 mm2 vs. 25.9 mm2 p < 0.001) but there was a more pronounced reduction in vascularized retinal area (67% vascularized vs. 54% vascularized, p < 0.001). Postnatal vascularization of the normal rat retina may be influenced by litter size.

摘要

许多视网膜疾病的动物模型使用新生大鼠。研究表明，在大窝中饲养幼鼠会导致出生后生长发育迟缓。我们研究了窝仔数对新生大鼠视网膜正常出生后血管形成的影响。66只新生大鼠幼崽被5只哺乳母鼠分为3个小窝（n = 10）和2个大窝（n = 18）。在出生后第6天处死大鼠，分析视网膜总面积和血管化视网膜面积。在大窝中饲养的大鼠视网膜总面积减少（28.6平方毫米对25.9平方毫米，p < 0.001），但血管化视网膜面积减少更为明显（血管化比例67%对54%，p < 0.001）。正常大鼠视网膜的出生后血管形成可能受窝仔数影响。