Shackney S E, Shankey T V
Laboratory of Cancer Cell Biology and Genetics, Allegheny-Singer Research Institute, Pittsburgh, PA, USA.
Cytometry. 1995 Sep 1;21(1):2-5. doi: 10.1002/cyto.990210103.
The presence of cellular heterogeneity within human tumors has been recognized for many years. Current concepts regarding the clonal origin of human neoplasms, and recent advances in the study of successive genetic changes that occur during tumor evolution may now make it possible to understand in greater depth the biological and clinical implications of intra-tumor heterogeneity at both the phenotypic and genotypic levels. In order to explore these concepts further, and to better identify the potential contributions that flow and image cytometry can make to our understanding of tumor heterogeneity, a session of the 1994 ISAC Congress was dedicated to plenary presentations on human cancer cell heterogeneity. Here, we provide a brief overview of the genetic evolutionary progression of human cancers, some considerations of clinically important phenotypic and genotypic markers, and an outline that might serve as a basis for framing relevant issues that are ammenable to further study. All Nature is but Art, unknown to thee; All Chance, Direction, which thou canst not see; All Discord, Harmony not understood: All partial Evil, universal Good. (Alexander Pope, Essay on Man, end of Epistle 1).
人类肿瘤中细胞异质性的存在已被认识多年。目前关于人类肿瘤克隆起源的概念，以及肿瘤进化过程中发生的连续基因变化研究的最新进展，现在可能使我们能够更深入地理解肿瘤内异质性在表型和基因型水平上的生物学和临床意义。为了进一步探讨这些概念，并更好地确定流式细胞术和图像细胞术对我们理解肿瘤异质性可能做出的潜在贡献，1994年国际细胞分析学会（ISAC）大会的一个分会专门进行了关于人类癌细胞异质性的全会报告。在此，我们简要概述人类癌症的基因进化进程，对临床重要的表型和基因型标志物进行一些思考，并给出一个大纲，可作为构建适合进一步研究的相关问题的基础。一切自然不过是你所不知的艺术；一切偶然皆为你所不见的方向；一切不和谐，都是你所不解的和谐；一切局部的恶，都是普遍的善。（亚历山大·蒲柏，《人论》，书信一结尾）
