采用场反转毛细管电泳进行DNA分离。

DNA separation with field inversion capillary electrophoresis.

作者信息

Heller C, Pakleza C, Viovy J L

机构信息

Laboratoire de Physique des Surfaces et Interfaces (URA CNRS 1379), Institut Curie, Paris, France.

出版信息

Electrophoresis. 1995 Aug;16(8):1423-8. doi: 10.1002/elps.11501601235.

DOI:10.1002/elps.11501601235

PMID:8529608

Abstract

We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double-stranded DNA in the range of 1-50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.

摘要

我们在一款商用设备的基础上构建了一个自动化脉冲场毛细管电泳系统。使用缠结聚合物溶液作为分离基质，我们证明了在脉冲场条件下，1 - 50 kbp范围内双链DNA的分离有显著改善。研究了主要参数即脉冲频率和电场的影响，并将结果与现有的电泳理论进行了比较。