Ritz P, Coward W A
Laboratoire de Nutrition Humaine, Centre de Recherche en Nutrition Humaine, Clermont-Ferrand, France.
Diabete Metab. 1995 Oct;21(4):241-51.
The doubly labelled water method uses the principles of indirect calorimetry to measure total energy expenditure from the turnover rates of two stable isotopes: deuterium and oxygen 18. Labelling total body water also provides estimates of body composition and measurements of water outflow rates. Although the principle of the method was determined in the 1950s, it was only applied to humans in the 1980s. Some 15 years later, it is time for an objective appraisal of the method. This review first describes the principle and practice of the doubly labelled water method. The original concept described by Lifson and MacClintock is then discussed, and proposals are made to adapt the method to physiological and pathophysiological situations.
双标记水法利用间接量热法原理，通过两种稳定同位素——氘和氧-18的转换率来测量总能量消耗。标记全身水分还可提供身体成分的估计值以及水流出率的测量值。尽管该方法的原理在20世纪50年代就已确定，但直到20世纪80年代才应用于人体。大约15年后，是时候对该方法进行客观评估了。本综述首先描述双标记水法的原理和实践。然后讨论了利夫森和麦克林托克最初提出的概念，并提出了使该方法适用于生理和病理生理情况的建议。
