Hatziloukas E, Panopoulos N J, Delis S, Prosen D E, Schaad N W
Department of Environmental Science, Policy and Management, University of California, Berkeley 94720, USA.
Gene. 1995 Dec 1;166(1):83-7. doi: 10.1016/0378-1119(95)00569-5.
Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.
丁香假单胞菌菜豆致病变种（Psp）的tox-argK基因簇中一个明显的开放阅读框的一部分可能编码一种与脂肪酸去饱和酶（DES）序列相似的多肽。在T7启动子控制下表达该片段的大肠杆菌B产生了一种34 kDa的多肽。本文讨论了DES在促进菜豆毒素（Ptx）在其合成所需的低温下分泌方面的可能作用，以及关于tox-argK基因簇起源的进化意义。
