4-羟基壬烯醛在大鼠肾脏中降解为硫醚氨酸共轭物。

4-hydroxynonenal is degraded to mercapturic acid conjugate in rat kidney.

作者信息

Petras T, Siems W G, Grune T

机构信息

Clinics of Physical Therapy and Rehabilitation, Medical Faculty (Charité), Humboldt-University Berlin, Germany.

出版信息

Free Radic Biol Med. 1995 Nov;19(5):685-8. doi: 10.1016/0891-5849(95)00060-b.

DOI:10.1016/0891-5849(95)00060-b

PMID:8529929

Abstract

The cytotoxic lipid peroxidation product 4-hydroxynonenal (HNE) was infused into rat kidney. During the first 2 min a rapid degradation of a 100 microM HNE solution was demonstrated. After 5 min the consumption rate of 4-HNE reached a steady state of about 75 nmoles/ml. The total HNE consumption rate was about 200 nmoles/g w.w./min. The excretion rate into urine was about 0.1% of total HNE consumption. It could be demonstrated that the HNE-mercapturic acid formation takes place in the kidney. The formation of the HNE-mercapturic acid contributes up to 6% to total HNE consumption. Within 10 min of perfusion 2% of the HNE-mercapturic acid were excreted into urine. The residual 98% flow back into the blood circulation.

摘要

将细胞毒性脂质过氧化产物4-羟基壬烯醛（HNE）注入大鼠肾脏。在最初2分钟内，证明100微摩尔HNE溶液迅速降解。5分钟后，4-HNE的消耗速率达到约75纳摩尔/毫升的稳定状态。HNE的总消耗速率约为200纳摩尔/克湿重/分钟。尿液排泄率约为HNE总消耗量的0.1%。可以证明，HNE-巯基尿酸的形成发生在肾脏中。HNE-巯基尿酸的形成占HNE总消耗量的比例高达6%。灌注10分钟内，2%的HNE-巯基尿酸排泄到尿液中。其余98%回流到血液循环中。