Song Z H, Modi W, Bonner T I
Laboratory of Cell Biology, NIMH, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genomics. 1995 Jul 20;28(2):347-9. doi: 10.1006/geno.1995.1154.
Cosmids containing human genes for three orphan G protein-coupled receptors, GPR12, GPR6, and GPR3, were isolated using their rat homologs as probes. Previous studies of the mouse and rat cDNAs have shown the receptors to be expressed primarily in brain but have failed to identify their ligands. The three receptor proteins of 334, 363, and 330 amino acids, respectively, are encoded by a single exon in each gene. Excluding the divergent sequences preceding the first transmembrane domain, they have approximately 60% amino acid identity with each other. Fluorescence in situ hybridization of GPR12, GPR6, and GPR3 localized these three genes to human chromosomal regions 13q12, 6q21, and 1p34.3-p36.1, respectively.
利用大鼠同源基因作为探针，分离出了包含人类三种孤儿G蛋白偶联受体（GPR12、GPR6和GPR3）基因的黏粒。先前对小鼠和大鼠cDNA的研究表明，这些受体主要在大脑中表达，但尚未确定它们的配体。这三种受体蛋白分别由334、363和330个氨基酸组成，每个基因均由一个外显子编码。除了第一个跨膜结构域之前的差异序列外，它们彼此之间的氨基酸同一性约为60%。GPR12、GPR6和GPR3的荧光原位杂交分别将这三个基因定位到人类染色体区域13q12、6q21和1p34.3 - p36.1。
