人类葡萄糖磷酸异构酶基因（GPI）的结构与组织

Structure and organization of the human glucose phosphate isomerase gene (GPI).

作者信息

Walker J I, Morgan M J, Faik P

机构信息

Wellcome Research Laboratory for Molecular Genetics, Division of Biochemistry and Molecular Biology, UMDS, Guy's Hospital, London, United Kingdom.

出版信息

Genomics. 1995 Sep 1;29(1):261-5. doi: 10.1006/geno.1995.1241.

DOI:10.1006/geno.1995.1241

PMID:8530082

Abstract

Two overlapping yeast artificial chromosome clones containing the human glucose 6-phosphate isomerase gene (GPI) have been isolated. PCR and direct sequencing were used to determine the exon/intron structure of the gene. The gene spans in excess of 40 kb and consists of 18 exons ranging in size from 44 to 153 bp. All splice sites conform to the GT/AG rule.

摘要

已分离出两个包含人类葡萄糖 6 - 磷酸异构酶基因（GPI）的重叠酵母人工染色体克隆。采用聚合酶链反应（PCR）和直接测序来确定该基因的外显子/内含子结构。该基因跨度超过40kb，由18个外显子组成，大小从44bp到153bp不等。所有剪接位点均符合GT/AG规则。