Daimon M, Yamatani K, Igarashi M, Fukase N, Kawanami T, Kato T, Tominaga M, Sasaki H
Third Department of Internal Medicine, Yamagata University, School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 Mar 28;208(3):1028-35. doi: 10.1006/bbrc.1995.1437.
We characterized the genomic region corresponding to the human ceruloplasmin cDNA previously reported. Using PCR-direct sequencing methods, we determined precise intron/exon boundaries and intron-exon composition of the gene in the region. The gene region spanned about 50 kb and was composed of 19 exons and 18 introns. The lengths of exons and introns range from 107 to over 267 bp and from 0.44 to 10.0 kb, respectively. The translation initiation codon and the termination codon were located in exons 1 and 19, respectively. The nucleotide sequences of the introns were also determined in the region around the intron/exon boundaries for 24-220 bp. All the sequences around the intron/exon boundaries were consistent with the 5' and 3' consensus sequences for splice junctions of transcribed genes. Putative lariat sequences were identified between -17 and -42 nucleotides from the 3' splice junction for all 18 introns.
我们对先前报道的与人类铜蓝蛋白cDNA相对应的基因组区域进行了特征分析。使用聚合酶链反应直接测序方法，我们确定了该区域基因精确的内含子/外显子边界和内含子-外显子组成。该基因区域跨度约50 kb，由19个外显子和18个内含子组成。外显子和内含子的长度分别为107至超过267 bp以及0.44至10.0 kb。翻译起始密码子和终止密码子分别位于外显子1和19中。还测定了内含子/外显子边界周围24 - 220 bp区域的内含子核苷酸序列。内含子/外显子边界周围的所有序列均与转录基因剪接连接的5'和3'共有序列一致。在所有18个内含子的3'剪接连接下游- 17至- 42个核苷酸之间鉴定出了假定的套索序列。
