血清中丙酮酸和α-酮戊二酸的高效液相色谱测定法
High-performance liquid chromatographic determination of pyruvic acid and alpha-ketoglutaric acid in serum.
作者信息
Terada H, Hayashi T, Kawai S, Ohno T
出版信息
J Chromatogr. 1977 Jan 11;130:281-6. doi: 10.1016/s0021-9673(00)89806-4.
PMID:853078
Abstract
A high-performance liquid chromatographic determination of pyruvic acid and alpha-ketoglutaric acid in serum is described which is based on the formation of 2,4-dinitrophenylhydrazones. The syn-anti isomerization of the 2,4-dinitrophenylhydrazone of pyruvic acid is also discussed.
摘要
本文描述了一种基于2,4-二硝基苯腙形成的血清中丙酮酸和α-酮戊二酸的高效液相色谱测定方法。同时还讨论了丙酮酸的2,4-二硝基苯腙的顺反异构化。
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