Liao J C, Hoffman N E, Barboriak J J, Roth D A
Clin Chem. 1977 May;23(5):802-5.
Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.
通过一种新方法，丙酮酸和α-酮戊二酸可被快速定量转化为喹喔啉酮，以提高它们在紫外线下的可检测性。使用填充有10微米颗粒的30厘米（长度）柱，通过反相色谱法分离喹喔啉酮。用乙酸铵水溶液和甲醇进行线性梯度洗脱。测定了正常个体、糖尿病患者和肾功能不全患者尿液中丙酮酸和α-酮戊二酸的排泄情况。
