Yamashita I, Atsuta Y, Shimazaki S, Miyatsu M
Yamashita Orthopaedic Clinic, Hokkaido, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1995 Sep;69(9):735-43.
We studied effects of prostaglandin E2 and sodium hyaluronate on the reactive muscle activity induced by intra-articular injection of bradykinin into the knee joint of rat. An animal model was developed to evaluate knee joint pain. In unanesthetized and spinalized rat, bradykinin (BK) was injected into the knee joint and the duration of the reactive activity of the knee flexor was evaluated. The duration of the reaction was elongated dependent on the injected BK dosage (0.025-2.5 micrograms). When prostaglandin E2 (PG E2) was injected with BK, the duration of the reaction increased significantly (p < 0.01). In contrast, sodium hyaluronate (Na-HA: average MW 900 kD) injected into the joint 3-24 hours in advance significantly inhibited the BK induced reaction (p < 0.05-0.01). A histological study demonstrated the fluorescein-labeled Na-HA diffusely infiltrating into the surface of the soft tissue. From these results, the animal model used in the present study was valuable to evaluate quantitatively knee joint pain. Furthermore, the sensitizing effect of PG E2 and the inhibitory effect of Na-HA on joint pain was objectively demonstrated.
我们研究了前列腺素E2和透明质酸钠对大鼠膝关节内注射缓激肽所诱导的反应性肌肉活动的影响。建立了一种动物模型来评估膝关节疼痛。在未麻醉和脊髓横断的大鼠中，将缓激肽（BK）注入膝关节，并评估膝关节屈肌反应性活动的持续时间。反应持续时间随注入的BK剂量（0.025 - 2.5微克）而延长。当前列腺素E2（PG E2）与BK一起注射时，反应持续时间显著增加（p < 0.01）。相反，提前3 - 24小时将透明质酸钠（Na - HA：平均分子量900 kD）注入关节可显著抑制BK诱导的反应（p < 0.05 - 0.01）。组织学研究表明，荧光素标记的Na - HA弥漫性浸润到软组织表面。从这些结果来看，本研究中使用的动物模型对于定量评估膝关节疼痛具有重要价值。此外，客观地证明了PG E2的致敏作用和Na - HA对关节疼痛的抑制作用。
