念珠菌属细胞外天冬氨酸蛋白酶的pH依赖性变性

pH-dependent denaturation of extracellular aspartic proteinases from Candida species.

作者信息

Wagner T, Borg von Zepelin M, Rüchel R

机构信息

Department of Medical Microbiology, University of Göttingen, Germany.

出版信息

J Med Vet Mycol. 1995 Jul-Aug;33(4):275-8.

PMID:8531028

Abstract

The yeasts Candida albicans. Candida tropicalis, and Candida parapsilosis secrete aspartic proteinases which may enhance virulence. Profiles of pH-dependent irreversible denaturation of such enzymes were determined at 37 degrees C. C. albicans proteinases from both serotypes A and B maintained 50% of their activity near pH 7.25. Proteinases from C. parapsilosis and C. tropicalis lost 50% of their activity at pH 6.75 and pH 6.15, respectively. This suggests that in the infected host only proteinases of C. albicans maintain a native state for any length of time.

摘要

白色念珠菌、热带念珠菌和近平滑念珠菌分泌的天冬氨酸蛋白酶可能会增强其毒力。在37摄氏度下测定了这些酶的pH依赖性不可逆变性曲线。A和B两种血清型的白色念珠菌蛋白酶在pH 7.25附近保持其50%的活性。近平滑念珠菌和热带念珠菌的蛋白酶分别在pH 6.75和pH 6.15时失去其50%的活性。这表明在受感染的宿主体内，只有白色念珠菌的蛋白酶能在任何时间段内保持天然状态。