IgM class autoantibodies in human cord serum.

It has previously been shown that human neonatal B lymphocytes in vitro can synthesize autoantibodies which typically are of IgM class, polyreactive and of low affinity and thought to represent natural autoantibodies. We screened 1034 cord serum specimens to find sera with elevated IgM levels; 98 such sera were further examined by an enzyme-linked immunosorbent assay (ELISA) to determine whether antibodies against a panel of autoantigens could be found. We detected low levels of IgM class antibodies against cardiolipin, pyruvate dehydrogenase, single-stranded DNA, thyroglobulin and Fc fragments of IgG as rheumatoid factors. The IgM concentration correlated with autoantibody levels. The specificity of the autoantibody assays was confirmed by inhibition tests. We conclude that several types of autoantibodies can be found in some sera of newborns and that increased IgM concentration of some neonates may reflect a polyclonal antibody response.