Franco G R, Simpson A J, Pena S D
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas-UFMG, Belo Horizonte, Brasil.
Mem Inst Oswaldo Cruz. 1995 Mar-Apr;90(2):215-6. doi: 10.1590/s0074-02761995000200015.
We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposited in DNA or protein databases. Transcripts with sequences without matches in the databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
我们基于表达序列标签（ESTs）技术启动了一项曼氏血吸虫基因发现计划，即从自动DNA序列单次测序获得的cDNA部分序列。ESTs可用于根据其与DNA或蛋白质数据库中其他物种序列的同源性来鉴定基因。数据库中无匹配序列的转录本可能代表新的寄生虫特异性基因。这种方法已证明非常有效，在不到两年的时间里已确定了大量新基因，使已知的曼氏血吸虫基因数量增加了一倍多。
