Franco G R, Adams M D, Soares M B, Simpson A J, Venter J C, Pena S D
Departamento de Bioquímica, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Gene. 1995 Jan 23;152(2):141-7. doi: 10.1016/0378-1119(94)00747-g.
A directional size-selected cDNA library constructed from Schistosoma mansoni (Sm) adult worm RNA was used for the generation of expressed sequence tags (EST). From one or both ends of 429 distinct cDNA clones 607 EST were obtained. Of these, only 16% were previously known Sm genes. More than 22% of the clones had matches with entries for other organisms in the databases. These new Sm genes constituted a broad range of transcripts distributed among cytoplasmic structural and regulatory proteins, enzymes, membrane, nuclear and secretory proteins, and proteins with other functions. Almost 33% of the clones had no significant database matches and thus potentially represent Sm-specific genes. Among the latter, several clones, as judged by their redundancy in the library, appear to represent abundant transcripts. The data, taken as a whole, more than double the number of Sm genes identified by nucleotide sequencing and indicate the potential value of the adoption of genome sequencing strategies for the rapid increase in knowledge of complex disease-causing organisms.
利用从曼氏血吸虫（Sm）成虫RNA构建的定向大小选择cDNA文库来生成表达序列标签（EST）。从429个不同的cDNA克隆的一端或两端获得了607个EST。其中，只有16%是先前已知的Sm基因。超过22%的克隆与数据库中其他生物的条目相匹配。这些新的Sm基因构成了广泛的转录本，分布在细胞质结构和调节蛋白、酶、膜、核和分泌蛋白以及具有其他功能的蛋白中。几乎33%的克隆在数据库中没有显著匹配，因此可能代表Sm特异性基因。在后者中，根据它们在文库中的冗余性判断，有几个克隆似乎代表丰富的转录本。总体而言，这些数据使通过核苷酸测序鉴定的Sm基因数量增加了一倍多，并表明采用基因组测序策略对于快速增加对复杂致病生物的了解具有潜在价值。
