Garcia M M, Brown H E, Harlan R E
Department of Otolaryngology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
Brain Res. 1995 Sep 18;692(1-2):23-40. doi: 10.1016/0006-8993(95)00625-z.
Injection of morphine (10 mg/kg) induced a complex immediate-early gene response in the rat forebrain, as detected with immunocytochemistry. The c-Fos protein was induced consistently in the dorsomedial caudate-putamen, the nucleus accumbens, and in midline and intralaminar nuclei of the thalamus. In some rats induction was also seen in the parietal and insular cortex and in lateral regions of the caudate-putamen. Induction was detectable, although weak, at 30 min, was maximal at 2 h, and was undetectable 3 h after injection. JunB was induced in the same regions of the caudate-putamen as found for c-Fos, but was not induced in the nucleus accumbens or thalamus. In the caudate-putamen, JunB induction was still present 3 h after injection. A considerably smaller induction of c-Jun was noted in the dorsomedial caudate-putamen and in deep neocortex. Expression of JunD was inhibited in intralaminar and midline thalamic nuclei. Increases in numbers of cells immunoreactive for a Jun-related antigen (Jra) were found in the caudate-putamen and nucleus accumbens. These results indicate a complex immediate-early gene response to acute morphine, suggesting that morphine activates or inhibits specific neurons and circuits in the forebrain.
通过免疫细胞化学检测发现，注射吗啡（10毫克/千克）可在大鼠前脑诱导出复杂的即刻早期基因反应。c-Fos蛋白在背内侧尾状核-壳核、伏隔核以及丘脑的中线和板内核中持续被诱导表达。在一些大鼠中，顶叶和岛叶皮质以及尾状核-壳核的外侧区域也出现了诱导现象。注射后30分钟可检测到诱导现象，尽管较弱，2小时时达到最大值，3小时后则检测不到。JunB在尾状核-壳核中与c-Fos相同的区域被诱导表达，但在伏隔核或丘脑中未被诱导。在尾状核-壳核中，注射后3小时JunB诱导现象仍然存在。在背内侧尾状核-壳核和深层新皮质中，c-Jun的诱导程度明显较小。JunD的表达在板内和中线丘脑核中受到抑制。在尾状核-壳核和伏隔核中发现与Jun相关抗原（Jra）免疫反应阳性的细胞数量增加。这些结果表明对急性吗啡存在复杂的即刻早期基因反应，提示吗啡激活或抑制了前脑中的特定神经元和神经回路。
