一种检测IgG类风湿因子的简单方法。

A simple method for detection of IgG rheumatoid factor.

作者信息

Riera C M, Risemberg A, Strusberg A, Yantorno C

出版信息

J Immunol Methods. 1977;15(3):223-8. doi: 10.1016/0022-1759(77)90059-x.

Abstract

A simple method for detection of IgG rheumatoid factor (RF) in sera and joint fluid is described. The technique is based on the action of 0.1 M 2-mercaptoethanol mixed directly with the material. After 2 h of incubation determinations of human anti-IgG were performed by latex agglutination test. Comparison with data obtained by using the conventional method, sequential 24 h treatment with 0.1 M 2-mercaptoethanol and 0.01 M iodoacetamide, shows similar results for both methods. A correlation was observed between the presence of IgGRF in synovial fluid and a severe clinical course with invalidating forms in patients suffering rheumatoid arthritis.

摘要

本文描述了一种检测血清和关节液中IgG类风湿因子（RF）的简单方法。该技术基于将0.1M 2-巯基乙醇直接与样本混合的作用。孵育2小时后，通过乳胶凝集试验测定人抗IgG。与使用传统方法（用0.1M 2-巯基乙醇和0.01M碘乙酰胺连续处理24小时）获得的数据进行比较，两种方法结果相似。在类风湿关节炎患者中，观察到关节液中IgG RF的存在与严重临床病程及致残形式之间存在相关性。