Furness G, Trocola M
J Infect Dis. 1977 Apr;135(4):507-11. doi: 10.1093/infdis/135.4.507.
The growth requirements of Ureaplasma urealyticum strains T-McA, T-Pi, T-27, and T-207 were studied. All strains grew in T-broth containing horse serum. Only strain T-McA grew in broth containing serum fraction A, a finding that confirmed that ureaplasmas are not homogeneous and suggested that they can be divided into two species on the basis of their requirement for horse serum. The growth factors required by T-McA are arginine, cystine, methionine, and unidentified metabolites in serum fraction A and trypticase soy broth. The vitamins in Eagle's minimal essential medium are not essential metabolites for T-McA but enhanced its growth. Therefore, we recommend that these vitamins be incorporated in ureaplasma media and that the effect of supplementing media with arginine, cystine, and methionine for the primary isolation of ureaplasmas be ascertained.
对解脲脲原体菌株T-McA、T-Pi、T-27和T-207的生长需求进行了研究。所有菌株都能在含有马血清的T肉汤中生长。只有菌株T-McA能在含有血清组分A的肉汤中生长，这一发现证实了脲原体并非同质，并表明可根据它们对马血清的需求将其分为两个种。T-McA所需的生长因子是精氨酸、胱氨酸、蛋氨酸以及血清组分A和胰蛋白酶大豆肉汤中的未知代谢物。伊格尔氏最低必需培养基中的维生素并非T-McA的必需代谢物，但能促进其生长。因此，我们建议将这些维生素添加到脲原体培养基中，并确定补充精氨酸、胱氨酸和蛋氨酸对脲原体初次分离培养基的影响。
