解脲脲原体（T株支原体）血清型菌株的改良代谢抑制试验
Modified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma).
作者信息
Robertson J A, Stemke G W
出版信息
J Clin Microbiol. 1979 Jun;9(6):673-6. doi: 10.1128/jcm.9.6.673-676.1979.
Abstract
Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on continued incubation. Antisera against serotype 8 showed cross-reactions with types 2 and 4. The ninth strain, Vancouver, when tested by this method did not fit the recognized serotyping scheme and may represent a new serotype.
摘要
针对解脲脲原体8种公认血清型制备的抗血清，通过改良代谢抑制试验，使用微量滴定板中的溴百里酚蓝肉汤，检测其对同源和异源抗原的反应。该方法能给出清晰的终点判定，且在持续孵育时通常能保持。抗血清8型与2型和4型显示出交叉反应。通过该方法检测的第9株菌株温哥华株不符合公认的血清分型方案，可能代表一种新的血清型。
相似文献
1
Modified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma).解脲脲原体（T株支原体）血清型菌株的改良代谢抑制试验
J Clin Microbiol. 1979 Jun;9(6):673-6. doi: 10.1128/jcm.9.6.673-676.1979.
2
Serological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）对解脲脲原体菌株进行血清学鉴定。
J Clin Pathol. 1982 Apr;35(4):439-43. doi: 10.1136/jcp.35.4.439.
3
Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans.从人类分离出的解脲脲原体菌株的扩展血清分型方案。
J Clin Microbiol. 1982 May;15(5):873-8. doi: 10.1128/jcm.15.5.873-878.1982.
4
Serotyping of Ureaplasma urealyticum by immunoperoxidase assay.通过免疫过氧化物酶测定法对解脲脲原体进行血清分型。
J Clin Microbiol. 1981 Apr;13(4):670-6. doi: 10.1128/jcm.13.4.670-676.1981.
5
Modified colony indirect epifluorescence test for serotyping Ureaplasma urealyticum and an adaptation to detect common antigenic specificity.用于解脲脲原体血清分型的改良菌落间接荧光试验及检测共同抗原特异性的改进方法。
J Clin Microbiol. 1981 Nov;14(5):582-4. doi: 10.1128/jcm.14.5.582-584.1981.
6
Fourteen serotypes of ureaplasma urealyticum (T-mycoplasmas) demonstrated by the complement-dependent mycoplasmacidal test.通过补体依赖支原体杀菌试验证实了14种解脲脲原体血清型（T-支原体）。
Infection. 1980;8(4):152-5. doi: 10.1007/BF01639122.
7
Problems associated with serotyping strains of Ureaplasma urealyticum.与解脲脲原体菌株血清分型相关的问题。
Diagn Microbiol Infect Dis. 1985 Jul;3(4):311-20. doi: 10.1016/0732-8893(85)90005-7.
8
Enzyme-linked immunosorbent assay for detection of specific antibodies to Ureaplasma urealyticum serotypes.用于检测解脲脲原体血清型特异性抗体的酶联免疫吸附测定
J Clin Microbiol. 1984 Mar;19(3):421-6. doi: 10.1128/jcm.19.3.421-426.1984.
9
Development of an enzyme-linked immunosorbent assay for serotyping ureaplasma urealyticum strains using monoclonal antibodies.利用单克隆抗体开发用于解脲脲原体菌株血清分型的酶联免疫吸附测定法。
Clin Diagn Lab Immunol. 2001 Jan;8(1):52-7. doi: 10.1128/CDLI.8.1.52-57.2001.
10
Comparison of growth inhibition and immunofluorescence tests in serotyping clinical isolates of Ureaplasma urealyticum.解脲脲原体临床分离株血清型鉴定中生长抑制试验与免疫荧光试验的比较
Br J Vener Dis. 1977 Jun;53(3):186-9. doi: 10.1136/sti.53.3.186.
引用本文的文献
1
Comparative genome analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains.19 株解脲脲原体和人型支原体的比较基因组分析。
BMC Microbiol. 2012 May 30;12:88. doi: 10.1186/1471-2180-12-88.
2
Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes.解脲脲原体中的广泛水平基因转移对其用于诊断目的的血清型分型提出了质疑。
J Clin Microbiol. 2011 Aug;49(8):2818-26. doi: 10.1128/JCM.00637-11. Epub 2011 Jun 22.
3
Detection and characterization of human Ureaplasma species and serovars by real-time PCR.实时 PCR 检测和鉴定人型脲原体种及血清型。
J Clin Microbiol. 2010 Aug;48(8):2715-23. doi: 10.1128/JCM.01877-09. Epub 2010 Jun 16.
4
Production of recombinant antigens of Ureaplasma parvum serotypes 3 and 6 for development of a serological assay.产脲脲原体3型和6型重组抗原用于血清学检测方法的开发。
Clin Vaccine Immunol. 2008 Mar;15(3):447-51. doi: 10.1128/CVI.00379-07. Epub 2007 Dec 19.
5
Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes).柔膜菌纲（无壁菌门）新物种描述的修订最低标准。
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2703-2719. doi: 10.1099/ijs.0.64722-0.
6
Development of a monoclonal antibody to a Ureaplasma urealyticum serotype 9 antigen.针对解脲脲原体9型血清型抗原的单克隆抗体的研制。
J Clin Microbiol. 1998 Apr;36(4):1125-7. doi: 10.1128/JCM.36.4.1125-1127.1998.
7
Polymerase chain reaction using 16S rRNA gene sequences distinguishes the two biovars of Ureaplasma urealyticum.使用16S rRNA基因序列的聚合酶链反应可区分解脲脲原体的两个生物变种。
J Clin Microbiol. 1993 Apr;31(4):824-30. doi: 10.1128/jcm.31.4.824-830.1993.
8
Fourteen serotypes of ureaplasma urealyticum (T-mycoplasmas) demonstrated by the complement-dependent mycoplasmacidal test.通过补体依赖支原体杀菌试验证实了14种解脲脲原体血清型（T-支原体）。
Infection. 1980;8(4):152-5. doi: 10.1007/BF01639122.
9
Standardized method for determining antimicrobial susceptibility of strains of Ureaplasma urealyticum and their response to tetracycline, erythromycin, and rosaramicin.解脲脲原体菌株抗菌药敏性测定的标准化方法及其对四环素、红霉素和罗沙米星的反应
Antimicrob Agents Chemother. 1981 Jul;20(1):53-8. doi: 10.1128/AAC.20.1.53.
10
Serological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）对解脲脲原体菌株进行血清学鉴定。
J Clin Pathol. 1982 Apr;35(4):439-43. doi: 10.1136/jcp.35.4.439.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Occurrence of urease in T strains of Mycoplasma.脲酶在支原体T株中的存在情况。
J Bacteriol. 1967 May;93(5):1513-20. doi: 10.1128/jb.93.5.1513-1520.1967.
3
Color test for the measurement of antibody to T-strain mycoplasmas.用于检测T株支原体抗体的颜色试验。
J Bacteriol. 1966 Jul;92(1):6-12. doi: 10.1128/jb.92.1.6-12.1966.
4
Relationships between mycoplasma and the etiology of nongonococcal urethritis and Reiter's syndrome.支原体与非淋菌性尿道炎及赖特综合征病因之间的关系。
Ann N Y Acad Sci. 1967 Jul 28;143(1):501-4. doi: 10.1111/j.1749-6632.1967.tb27694.x.
5
Serologic typing of human genital T-mycoplasmas by a complement-dependent mycoplasmacidal test.采用补体依赖支原体杀菌试验对人泌尿生殖道T支原体进行血清学分型。
J Infect Dis. 1972 Dec;126(6):658-63. doi: 10.1093/infdis/126.6.658.
6
Non-specific urethritis associated with a tetracycline-resistant T-mycoplasma.与四环素耐药性T支原体相关的非特异性尿道炎。
Br J Vener Dis. 1974 Oct;50(5):373-4. doi: 10.1136/sti.50.5.373.
7
Application of indirect immunofluorescence, indirect haemagglutination and polyacrylamide-gel electrophoresis to human T-mycoplasmas.间接免疫荧光、间接血凝和聚丙烯酰胺凝胶电泳在人T支原体中的应用。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):345-53. doi: 10.1111/j.1699-0463.1974.tb02336.x.
8
Heat lability of rabbit anti-Mycoplasma pneumoniae serum.兔抗肺炎支原体血清的热稳定性
Antonie Van Leeuwenhoek. 1972;38(2):193-200. doi: 10.1007/BF02328091.
9
Serum antibody levels against T mycoplasmas in two North American Indian populations predisposed to spondylitis.两个易患脊柱炎的北美印第安人群中针对T支原体的血清抗体水平。
Arthritis Rheum. 1976 Nov-Dec;19(6):1328-32. doi: 10.1002/art.1780190614.
10
Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons.解脲脲原体八种血清型在非淋菌性尿道炎、淋病患者及健康人群中的分布情况。
Br J Vener Dis. 1976 Aug;52(4):266-8. doi: 10.1136/sti.52.4.266.
Zotero 插件