Ukena K, Oumi T, Matsushima O, Ikeda T, Fujita T, Minakata H, Nomoto K
Faculty of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Peptides. 1995;16(6):995-9. doi: 10.1016/0196-9781(95)00071-q.
A novel bioactive tetradecapeptide, GFKDGAADRISHGFamide, was isolated from the gut of the oligochaete annelid, Eisenia foetida, using the isolated anterior gut (crop-gizzard) as a bioassay system. A highly homologous peptide, GFRDGSADRISHGFamide, was also purified from the whole body of another species of earthworm, Pheretima vittata. These peptides were termed Eisenia tetradecapeptide (ETP) and Pheretima tetradecapeptide (PTP), respectively. Both the peptides showed a potent excitatory action on spontaneous contractions of the anterior gut with a threshold as low as 10(-10)-10(-9) M. These peptides were significantly homologous to molluscan tetradecapeptides and, to a lesser extent, to arthropodan tridecapeptides that have been reported to date. All these peptides seem to be evolutionally related to each other.
利用分离的前肠（嗉囊-砂囊）作为生物测定系统，从寡毛纲环节动物赤子爱胜蚓的肠道中分离出一种新型生物活性十四肽，即GFKDGAADRISHGFamide。还从另一种蚯蚓参环毛蚓的整体中纯化出一种高度同源的肽，即GFRDGSADRISHGFamide。这些肽分别被命名为爱胜蚓十四肽（ETP）和参环毛蚓十四肽（PTP）。这两种肽对前肠的自发收缩均表现出强烈的兴奋作用，阈值低至10(-10)-10(-9) M。这些肽与迄今为止报道的软体动物十四肽具有显著同源性，在较小程度上与节肢动物十三肽也有同源性。所有这些肽似乎在进化上彼此相关。
