Minakata H, Fujita T, Kawano T, Nagahama T, Oumi T, Ukena K, Matsushima O, Muneoka Y, Nomoto K
Suntory Institute for Bioorganic Research, Osaka, Japan.
FEBS Lett. 1997 Jun 30;410(2-3):437-42. doi: 10.1016/s0014-5793(97)00567-x.
A member of the GGNG peptide family was isolated from Hirudo nipponia (leech). GGNG peptides had only been isolated previously from earthworms. The C-terminus structure of the leech peptide, LEP (leech excitatory peptide), was -Gly-Gly-Asn-amide, while that of the earthworm peptides, EEP (earthworm excitatory peptide), was -Gly-Gly-Asn-Gly. LEP exerted 1000-fold more potent activities on leech gut than did EEP-2. On the other hand, EEP-2 was 1000-fold more potent than LEP on the crop-gizzard of the earthworm. Analog peptides of LEP and EEP-2 were synthesized, and the myoactive potency of each analog on the leech and earthworm tissues was compared.
从日本医蛭（水蛭）中分离出了GGNG肽家族的一个成员。此前，GGNG肽仅从蚯蚓中分离得到。水蛭肽LEP（水蛭兴奋性肽）的C端结构为-Gly-Gly-Asn-酰胺，而蚯蚓肽EEP（蚯蚓兴奋性肽）的C端结构为-Gly-Gly-Asn-Gly。LEP对水蛭肠道的活性比EEP-2强1000倍。另一方面，EEP-2对蚯蚓嗉囊-砂囊的活性比LEP强1000倍。合成了LEP和EEP-2的类似肽，并比较了每种类似物对水蛭和蚯蚓组织的肌活性效力。
