Hrycaj P, Wurm K, Mennet P, Müller W
Hochrhein Institute for Research and Prevention of Rheumatic Diseases, Bad Säckingen Germany/Rheinfelden, Switzerland.
Sarcoidosis. 1995 Sep;12(2):124-30.
Serum concentrations of IgG, IgA, and IgM antibodies to three heat shock proteins (HSPs)--ubiquitin, HSP70, and HSP90--were measured using ELISA in 37 patients with pulmonary sarcoidosis. When compared to healthy controls (n = 44), increased concentrations of IgA and IgG antibodies to ubiquitin were found in 13 (35.1%, p < 0.002) and 7 (18.9%, p < 0.05) patients, respectively. In 10 patients (27.0%) elevated concentrations of IgG antibodies to HSP70 were detected (p < 0.02), whereas IgA antibodies to this protein were found in 7 cases (18.9%, p < 0.05). IgM antibodies to ubiquitin and HSP70, and antibodies to HSP90 were not detected in patients' sera. The levels of antibodies to ubiquitin and HSP70 correlated well with each other within the given immunoglobulin class (r = 0.7391, p < 1E-5 and r = 0.9854, p < 1E-5 for IgG and IgA class, respectively). There was also a weak correlation between the level of IgG antibodies to HSP70 and both serum activity of angiotensin converting enzyme (SACE; r = 0.4668, p < 0.005) and serum level of soluble receptor for interleukin 2 (sIL-2-R; r = 0.4142), p < 0.02). A similar tendency was seen with IgG antibodies to ubiquitin. Furthermore, there was an association between the increased concentration of C-reactive protein (CRP) and increased levels of IgG antibodies to ubiquitin and HSP70 (p < 0.005 and p < 0.05, respectively). Our results suggest that antibodies to various HSPs are present in a subset of patients with sarcoidosis. The humoral immune response to HSPs relates probably to the immune activation and/or infection.
采用酶联免疫吸附测定法（ELISA）检测了37例肺结节病患者血清中针对三种热休克蛋白（HSPs）——泛素、HSP70和HSP90的IgG、IgA和IgM抗体浓度。与健康对照者（n = 44）相比，分别在13例（35.1%，p < 0.002）和7例（18.9%，p < 0.05）患者中发现泛素特异性IgA和IgG抗体浓度升高。在10例患者（27.0%）中检测到HSP70特异性IgG抗体浓度升高（p < 0.02），而7例（18.9%，p < 0.05）患者中发现了该蛋白的IgA抗体。患者血清中未检测到泛素和HSP70特异性IgM抗体以及HSP90抗体。在给定免疫球蛋白类别中，泛素和HSP70抗体水平彼此之间具有良好的相关性（IgG和IgA类别中，r分别为0.7391，p < 1E - 5和r = 0.9854，p < 1E - 5）。HSP70特异性IgG抗体水平与血管紧张素转换酶血清活性（SACE；r = 0.4668，p < 0.005）以及白细胞介素2可溶性受体血清水平（sIL - 2 - R；r = 0.4142，p < 0.02）之间也存在弱相关性。泛素特异性IgG抗体也有类似趋势。此外，C反应蛋白（CRP）浓度升高与泛素和HSP70特异性IgG抗体水平升高之间存在关联（分别为p < 0.005和p < 0.05）。我们的结果表明，结节病患者亚组中存在针对各种HSPs的抗体。对HSPs的体液免疫反应可能与免疫激活和/或感染有关。
